Isolated mitochondria are widely used to study the function of the organelle.Typically, mitochondria are prepared using differential centrifugation alone or in conjunction with density gradient ultracentrifugation.However, mitochondria isolated using differential centrifugation contain membrane or Chair Sash organelle contaminants, and further puri
For the analysis of thin films, with high aspect ratio (HAR) structures, time-of-flight secondary ion mass spectrometry (ToF-SIMS) overcomes several challenges in comparison to other frequently used techniques such as electron microscopy.The research presented herein focuses on two different kinds of HAR structures that represent different semicond
Mutations that give rise to premature termination codons are Sale a common cause of inherited genetic diseases.When transcripts containing these changes are generated, they are usually rapidly removed by the cell through the process of nonsense-mediated decay.Here we discuss observed changes in transcripts of the centrosomal protein CEP290 resultin
Stormwater runoff from carparks should be treated to remove pollutants before they enter urban waterways; however, differences in traffic characteristics and surrounding land use activities can result in varying first flush pollutant types and concentrations requiring specific treatment approaches.An understanding of potential variations in first f